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apoptosis antibody array kits (RayBiotech inc)

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RayBiotech inc apoptosis antibody array kits

Effect of KKA treatment on the expression of several proteins involved in the progression of apoptotic events. Heatmap characterizes signal strengths of each protein in the KKA treatment group and control group. Red bands in the cluster diagram show upregulation although green bands indicate downregulation of proteins. Values shown are mean ± SD of two experiments (n = 4 for every protein). * P < 0.05, ** P < 0.01, and *** P < 0.001 indicate significant differences in comparison with the amount of zero. KKA, Potassium koetjapate; TRAIL, tumor necrosis factor-related <t>apoptosis-inducing</t> ligand; Bcl-2, B-cell lymphoma-2; IGF1, insulin-like growth factor 1 receptor; IGFBP-6, insulin-like growth factor binding protein 6; CD40, cluster of differentiation 40; Hsp, heat shock protein.

Apoptosis Antibody Array Kits, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 94/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+apoptosis+antibody+array+kit/Human+Apoptosis+Signaling+Array+C1/pmc11257210-44-13-17
Average 94 stars, based on 38 article reviews

apoptosis antibody array kits - by Bioz Stars, 2026-10

94/100 stars

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1) Product Images from "Evaluation of in vitro and in vivo anticancer activities of potassium koetjapate: a solubility improved formulation of koetjapic acid against human colon cancer"

Article Title: Evaluation of in vitro and in vivo anticancer activities of potassium koetjapate: a solubility improved formulation of koetjapic acid against human colon cancer

Journal: Research in Pharmaceutical Sciences

doi: 10.4103/RPS.RPS_247_22

Figure Legend Snippet: Effect of KKA treatment on the expression of several proteins involved in the progression of apoptotic events. Heatmap characterizes signal strengths of each protein in the KKA treatment group and control group. Red bands in the cluster diagram show upregulation although green bands indicate downregulation of proteins. Values shown are mean ± SD of two experiments (n = 4 for every protein). * P < 0.05, ** P < 0.01, and *** P < 0.001 indicate significant differences in comparison with the amount of zero. KKA, Potassium koetjapate; TRAIL, tumor necrosis factor-related apoptosis-inducing ligand; Bcl-2, B-cell lymphoma-2; IGF1, insulin-like growth factor 1 receptor; IGFBP-6, insulin-like growth factor binding protein 6; CD40, cluster of differentiation 40; Hsp, heat shock protein.

Techniques Used: Expressing, Control, Comparison, Binding Assay

Ab Array:

Article Title: Specific Forms of Graphene Quantum Dots Induce Apoptosis and Cell Cycle Arrest in Breast Cancer Cells
Article Snippet: Cell cycle analysis was performed using the Muse ® Cell Cycle Kit and analyzed by the Muse Cell Analyzer (Luminex, Austin, TX, USA). .. The human apoptosis antibody array kit (AAH-APO-1; Ray Biotech, Peachtree Corners, GA, USA) was used to screen the apoptosis-related proteins. ..

Article Title: Cell Cycle Arrest and Apoptosis Induction via Modulation of Mitochondrial Integrity by Bcl-2 Family Members and Caspase Dependence in Dracaena cinnabari -Treated H400 Human Oral Squamous Cell Carcinoma
Article Snippet: .. Involvement of apoptotic proteins during apoptosis was determined through apoptosis array using Human Apoptosis Antibody Array kit (RayBiotech, GA, USA) according to the manufacturer's instructions. ..

Article Title: Fraisinib: a calixpyrrole derivative reducing A549 cell-derived NSCLC tumor in vivo acts as a ligand of the glycine-tRNA synthase, a new molecular target in oncology
Article Snippet: The cell cycle was then measured and determined following the manufacturer’s instructions using the Muse Cell Analyzer (Millipore Merck, Vimodrone MI, Italy). .. To detect simultaneously the relative levels of expression of many apoptosis-related proteins in a single sample, their quantification was carried out using a Human Apoptosis Antibody Array Kit (RayBiotech, GA, United States of America) following the manufacturer’s instructions. ..

Article Title: A triple-drug combination induces apoptosis in cervical cancer-derived cell lines
Article Snippet: .. The Human Apoptosis Antibody Array Kit (RayBiotech, Inc., Norcross, USA) was used to evaluate the apoptotic protein expression according to the manufacturer’s instructions. ..

Article Title: Inhibition of human prostate cancer (PC-3) cells and targeting of PC-3-derived prostate cancer stem cells with koenimbin, a natural dietary compound from Murraya koenigii (L) Spreng
Article Snippet: The PCR products were exposed to 1.5% agarose gel electrophoresis, then stained, and visualized under ultraviolet (UV) light using Gel Doc XR System (Bio-Rad Laboratories Inc., Hercules, CA, USA). .. PC-3 cells induced with 6 μg/mL of koenimbin for 48 h were compared to the control untreated PC-3 cells to determine the apoptotic-associated proteins using a proteome profiler array (human apoptosis antibody array kit; RayBiotech, Norcross, GA, USA), according to the manufacturer’s instructions. .. Briefly, 250 μg of extracted protein from each sample was incubated with antibody array membrane and was then quantified using a Biospectrum AC ChemiHR 40 system (UVP, Upland, CA, USA).

Article Title: Mechanistic Study of Triazole Based Aminodiol Derivatives in Leukemic Cells—Crosstalk between Mitochondrial Stress-Involved Apoptosis and Autophagy
Article Snippet: .. Apoptosis-related proteins were determined using an antibody array (human apoptosis antibody array kit, Raybiotech, Norcross, GA, USA). .. After the indicated treatment, the cells were collected in which the protein extract (300 μg) from each sample was loaded onto an antibody array membrane for 4 h. Each membrane was quantified using a Biospectrum AC ChemiHR 40 system, and the membrane image file was analyzed using a UVP analysis software (UVP, Upland, CA, USA).

Article Title: The Resveratrol Oligomers Cis- and Trans-gnetin H Inhibit Human Cancer Cells by Induction of Apoptosis and Oxidative Stress
Article Snippet: Cells were imaged using Arrayscan VTI High-Content Screening (HCS) reader (Thermo Scientific) with appropriate filters, and the data were analyzed using the vHCS Scan software. .. To evaluate apoptotic protein expression, The Human Apoptosis Antibody Array kit (RayBiotech, inc., Norcross GA, USA) was used. ..

Expressing:

Control:

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Pathway enrichment analysis for the 33 target genes of C. sappan L. ( A ) The Gene Ontology (GO) biological process (BP) gene sets, ( B ) GO cellular component (CC) gene sets, ( C ) GO molecular function (MF) gene sets, and ( D ) the Kyoto Encyclopedia of Genes and Genomes (KEGG) gene sets. ( E ) Pathways in cancer involved in cell cycle regulation, apoptosis, and signal transduction.

Journal: Plants

Article Title: An Investigation of the Anticancer Mechanism of Caesalpinia sappan L. Extract Against Colorectal Cancer by Integrating a Network Pharmacological Analysis and Experimental Validation

doi: 10.3390/plants14020263

Figure Lengend Snippet: Pathway enrichment analysis for the 33 target genes of C. sappan L. ( A ) The Gene Ontology (GO) biological process (BP) gene sets, ( B ) GO cellular component (CC) gene sets, ( C ) GO molecular function (MF) gene sets, and ( D ) the Kyoto Encyclopedia of Genes and Genomes (KEGG) gene sets. ( E ) Pathways in cancer involved in cell cycle regulation, apoptosis, and signal transduction.

Article Snippet: The Human Apoptosis Proteome Profiler Antibody Array kit (R&D Systems, Minneapolis, MN, USA) was used to simultaneously confirm the relative expression levels of multiple apoptosis-related proteins from HCT 116 and COLO 205 cells after CSE treatment (30 μg/mL).

Techniques: Transduction

Effect of CSE on apoptosis induction in HCT 116 and COLO 205 cells. ( A ) Flow cytometric analysis and apoptosis percentage of HCT 116 cells stained with Annexin V/7-AAD after 24 h and 48 h of CSE treatment. The apoptotic rate means the rate of Annexin V-positive cells. ( B ) The caspase 3/7 activity of HCT 116 cells was evaluated using the Caspase-Glo 3/7 Assay Kit after 48 h of CSE treatment. ( C ) Flow cytometric analysis and apoptosis percentage of COLO 205 cells stained with Annexin V/7-AAD after 24 h and 48 h of CSE treatment. The apoptotic rate means the rate of Annexin V-positive cells. ( D ) The caspase 3/7 activity of COLO 205 was evaluated using the Caspase-Glo 3/7 Assay Kit after 48 h of CSE treatment. Bars represent the mean ± SD (n = 3). ** p < 0.01 and *** p < 0.001, compared to the control (0 μg/mL) group.

Journal: Plants

doi: 10.3390/plants14020263

Figure Lengend Snippet: Effect of CSE on apoptosis induction in HCT 116 and COLO 205 cells. ( A ) Flow cytometric analysis and apoptosis percentage of HCT 116 cells stained with Annexin V/7-AAD after 24 h and 48 h of CSE treatment. The apoptotic rate means the rate of Annexin V-positive cells. ( B ) The caspase 3/7 activity of HCT 116 cells was evaluated using the Caspase-Glo 3/7 Assay Kit after 48 h of CSE treatment. ( C ) Flow cytometric analysis and apoptosis percentage of COLO 205 cells stained with Annexin V/7-AAD after 24 h and 48 h of CSE treatment. The apoptotic rate means the rate of Annexin V-positive cells. ( D ) The caspase 3/7 activity of COLO 205 was evaluated using the Caspase-Glo 3/7 Assay Kit after 48 h of CSE treatment. Bars represent the mean ± SD (n = 3). ** p < 0.01 and *** p < 0.001, compared to the control (0 μg/mL) group.

Techniques: Staining, Activity Assay, Caspase-Glo Assay, Control

Effect of CSE on apoptosis and cell cycle regulatory proteins in HCT 116 and COLO 205 cells. ( A ) Images of protein array showing the expression of apoptosis-related proteins in control and CSE-treated HCT 116 and COLO 205 cells. Cells were treated with 30 μg/mL CSE for 48 h. ( B ) Quantitative comparison of selected protein levels, shown as average fold-difference relative to control (0 μg/mL), based on duplicates from the protein array. ( C ) The expression levels of apoptotic proteins, including PARP, caspase-8, caspase-9, and caspase-3. ( D ) The expression levels of cell cycle regulatory proteins, including XIAP, cyclin D1, CDK2, p27, and p21. ( E ) Relative mRNA expression of cell-cycle-related genes in control and CSE-treated HCT 116 cells. Bars represent the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001, compared to the control group.

Journal: Plants

doi: 10.3390/plants14020263

Figure Lengend Snippet: Effect of CSE on apoptosis and cell cycle regulatory proteins in HCT 116 and COLO 205 cells. ( A ) Images of protein array showing the expression of apoptosis-related proteins in control and CSE-treated HCT 116 and COLO 205 cells. Cells were treated with 30 μg/mL CSE for 48 h. ( B ) Quantitative comparison of selected protein levels, shown as average fold-difference relative to control (0 μg/mL), based on duplicates from the protein array. ( C ) The expression levels of apoptotic proteins, including PARP, caspase-8, caspase-9, and caspase-3. ( D ) The expression levels of cell cycle regulatory proteins, including XIAP, cyclin D1, CDK2, p27, and p21. ( E ) Relative mRNA expression of cell-cycle-related genes in control and CSE-treated HCT 116 cells. Bars represent the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001, compared to the control group.

Techniques: Protein Array, Expressing, Control, Comparison

Transcriptomic effects of CSE treatment. ( A ) Volcano plot depicting differentially expressed genes (DEGs) between CSE-treated and control groups. Red points indicate significantly upregulated genes, while blue points represent significantly downregulated genes (|log 2 fold change (FC)| > 1.2, p -value < 0.05). ( B ) EnrichR analysis showing significantly enriched cellular processes. Key processes include apoptosis, cell cycle regulation, and immune response, based on the DEGs from the CSE treatment group. ( C ) Gene Set Enrichment Analysis (GSEA) plots demonstrating the impact of CSE on specific pathways. Cell cycle checkpoint signaling is significantly influenced, with both upregulation and downregulation observed across the ranked gene list. T-cell signaling pathways, including co-stimulation and regulation of apoptosis, are notably enriched. Pathways related to immune responses, such as interferon-mediated signaling and inflammasome assembly, show significant enrichment. ( D ) Drug–pathway–gene interaction network reconstructed using the GSEA results. The network highlights the interactions between CSE-regulated genes (circles), key pathways (squares), and drugs (hexagons). Node colors represent the log 2 fold change in gene expression, with connections indicating regulatory relationships: arrows signify positive regulation, T-shaped ends indicate negative regulation, and lines represent associations. Only genes with |FC| > 1.2 are included, aligned with the regulatory patterns of the pathways.

Journal: Plants

doi: 10.3390/plants14020263

Figure Lengend Snippet: Transcriptomic effects of CSE treatment. ( A ) Volcano plot depicting differentially expressed genes (DEGs) between CSE-treated and control groups. Red points indicate significantly upregulated genes, while blue points represent significantly downregulated genes (|log 2 fold change (FC)| > 1.2, p -value < 0.05). ( B ) EnrichR analysis showing significantly enriched cellular processes. Key processes include apoptosis, cell cycle regulation, and immune response, based on the DEGs from the CSE treatment group. ( C ) Gene Set Enrichment Analysis (GSEA) plots demonstrating the impact of CSE on specific pathways. Cell cycle checkpoint signaling is significantly influenced, with both upregulation and downregulation observed across the ranked gene list. T-cell signaling pathways, including co-stimulation and regulation of apoptosis, are notably enriched. Pathways related to immune responses, such as interferon-mediated signaling and inflammasome assembly, show significant enrichment. ( D ) Drug–pathway–gene interaction network reconstructed using the GSEA results. The network highlights the interactions between CSE-regulated genes (circles), key pathways (squares), and drugs (hexagons). Node colors represent the log 2 fold change in gene expression, with connections indicating regulatory relationships: arrows signify positive regulation, T-shaped ends indicate negative regulation, and lines represent associations. Only genes with |FC| > 1.2 are included, aligned with the regulatory patterns of the pathways.

Techniques: Control, Protein-Protein interactions, Gene Expression

A CCK-8 assay was used to test the proliferation of OS cells. Cell proliferation ability was evaluated by the OD value of CCK-8 assay at 450 nm. B Wound healing assay was performed to detect the migration ability of MNNG HOS and U-2OS cells (magnification: ×50). C The migratory ability of OS cells was measured by trans-well assay (magnification: ×200). The scale bar is 100 μm. D The apoptosis of OS cells after infection with shPOLE2s was determined by flow cytometry (FCM). E The Human Apoptosis Antibody Array was used to investigate the changes of cell apoptosis- related proteins in MNNG HOS cells after POLE2 knockdown. Histogram was used to show the proteins with significant changes. ShCtrl: cells infected with negative control shRNA; shPOLE2: cells infected with POLE2 shRNA. * P < 0.05, ** P < 0.01.

Journal: Cell Death Discovery

Article Title: POLE2 promotes osteosarcoma progression by enhancing the stability of CD44

doi: 10.1038/s41420-024-01875-x

Figure Lengend Snippet: A CCK-8 assay was used to test the proliferation of OS cells. Cell proliferation ability was evaluated by the OD value of CCK-8 assay at 450 nm. B Wound healing assay was performed to detect the migration ability of MNNG HOS and U-2OS cells (magnification: ×50). C The migratory ability of OS cells was measured by trans-well assay (magnification: ×200). The scale bar is 100 μm. D The apoptosis of OS cells after infection with shPOLE2s was determined by flow cytometry (FCM). E The Human Apoptosis Antibody Array was used to investigate the changes of cell apoptosis- related proteins in MNNG HOS cells after POLE2 knockdown. Histogram was used to show the proteins with significant changes. ShCtrl: cells infected with negative control shRNA; shPOLE2: cells infected with POLE2 shRNA. * P < 0.05, ** P < 0.01.

Article Snippet: Human Apoptosis Antibody Array Kit (Abcam, Cambridge, MA, USA) was analyzed to investigate the expression changes of proteins related to cell apoptosis.

Techniques: CCK-8 Assay, Wound Healing Assay, Migration, Infection, Flow Cytometry, Ab Array, Negative Control, shRNA

A The MTT assay was used for assessing the proliferation ability of MNNG HOS cells after overexpressing POLE2, knockdown CD44, or overexpressing POLE2 while knockdown CD44. B , C The invasive ability of MNNG HOS cells was determined by trans-well assay. D The apoptosis of MNNG HOS cells in POLE2 overexpression group, shCD44 group, and POLE2 + shCD44 group was detected by FCM. E , F The mRNA and protein expression of CD44 and MDM2 in tumor tissues of POLE2 knockdown nude mice models were detected by qPCR and western blot, respectively. POLE2: cells infected with POLE2 overexpression lentivirus; shCtrl: cells infected with negative control shRNA; shCD44, cells infected with CD44 shRNA; POLE2 + shCD44, cells co-infected with POLE2 overexpression vector and CD44 shRNA. * P < 0.05, ** P < 0.01, *** P < 0.001.

Journal: Cell Death Discovery

Article Title: POLE2 promotes osteosarcoma progression by enhancing the stability of CD44

doi: 10.1038/s41420-024-01875-x

Figure Lengend Snippet: A The MTT assay was used for assessing the proliferation ability of MNNG HOS cells after overexpressing POLE2, knockdown CD44, or overexpressing POLE2 while knockdown CD44. B , C The invasive ability of MNNG HOS cells was determined by trans-well assay. D The apoptosis of MNNG HOS cells in POLE2 overexpression group, shCD44 group, and POLE2 + shCD44 group was detected by FCM. E , F The mRNA and protein expression of CD44 and MDM2 in tumor tissues of POLE2 knockdown nude mice models were detected by qPCR and western blot, respectively. POLE2: cells infected with POLE2 overexpression lentivirus; shCtrl: cells infected with negative control shRNA; shCD44, cells infected with CD44 shRNA; POLE2 + shCD44, cells co-infected with POLE2 overexpression vector and CD44 shRNA. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Human Apoptosis Antibody Array Kit (Abcam, Cambridge, MA, USA) was analyzed to investigate the expression changes of proteins related to cell apoptosis.

Techniques: MTT Assay, Over Expression, Expressing, Western Blot, Infection, Negative Control, shRNA, Plasmid Preparation

Journal: Research in Pharmaceutical Sciences

Article Title: Evaluation of in vitro and in vivo anticancer activities of potassium koetjapate: a solubility improved formulation of koetjapic acid against human colon cancer

doi: 10.4103/RPS.RPS_247_22

Figure Lengend Snippet: Effect of KKA treatment on the expression of several proteins involved in the progression of apoptotic events. Heatmap characterizes signal strengths of each protein in the KKA treatment group and control group. Red bands in the cluster diagram show upregulation although green bands indicate downregulation of proteins. Values shown are mean ± SD of two experiments (n = 4 for every protein). * P < 0.05, ** P < 0.01, and *** P < 0.001 indicate significant differences in comparison with the amount of zero. KKA, Potassium koetjapate; TRAIL, tumor necrosis factor-related apoptosis-inducing ligand; Bcl-2, B-cell lymphoma-2; IGF1, insulin-like growth factor 1 receptor; IGFBP-6, insulin-like growth factor binding protein 6; CD40, cluster of differentiation 40; Hsp, heat shock protein.

Article Snippet: The materials, reagents, and instruments that used in this study were as follows: apoptosis antibody array kits (Raybiotech, Parkway, Norcross, GA, USA), betulinic acid (Fisher, USA), bovine serum albumin and fetal bovine serum (Chromadex, USA), human colorectal carcinoma (HCT 116, ATCC ® ), Cignal FinderTM 10-pathway reporter array system (Qiagen, Hilden, Germany), dual luciferase reporter system (Promega, Madison, USA), Matrigel (BD Bioscience.

Techniques: Expressing, Control, Comparison, Binding Assay

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apoptosis antibody array kits (RayBiotech inc)

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1) Product Images from "Evaluation of in vitro and in vivo anticancer activities of potassium koetjapate: a solubility improved formulation of koetjapic acid against human colon cancer"

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